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Methods: Disc specimens (6 x 3 mm) of resin ionomer (RelyX/3M ESPE), white copper cement (Doc's Best/Cooley & Cooley) and composite resin coated with and cured, dental adhesive (Clearfil Protect Bond/ Kuraray) were fabricated.. The specimens of each material were rinsed with sterile water stored in tubes of sterile medium at 35ºC for the predetermined time period of day 0, one month, and one year. At each time period, three tubes from each set was removed and the specimens for each material were transferred to test tubes containing 10 ml of Mitis Salivarius (DIFCO) medium. These were then inoculated each with either 0.1 ml 18-14 hr cultures of Streptococcus salivarius or Streptococcus mutans. The inoculated tubes were then incubated anaerobically at 35ºC for 48 hours. The tubes were sonicated, vortexed and suitably diluted and plated in duplicate on agar plates. Plates were incubated anaerobically at 35º C for 24 hrs and then incubated aerobically at 25ºC. Matching controls were run in tubes of media without the discs and treated similarly. Growth of each organism was determine and percent kill rate was calculated as compared to controls.
Results: Percent of bacterial kill rate of dental materials.
Streptococcus mutans % Kill | |||
Material | Day 0 | One Month | One Year |
Clearfil PB | 2.7 | 0 | 8.5 |
Rely X | 6.1 | 4.1 | 68.1 |
Doc's | 14.9 | 2.4 | 71.1 |
Streptococcus salivarius % Kill
Material | Day 0 | One Month | One Year |
Clearfil PB | 13.2 | 11.1 | 6.0 |
Rely X | 12.6 | 48.6 | 25.2 |
Doc's | 20 | 40.4 | 52.6 |
Conclusions: After one year, white copper cement showed the greatest percent bacterial kill rate among all test materials. Streptococcus mutans was more susceptible to these compounds.
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